Attenuation of Reproductive Dysfunctions by Hydroethanolic Leaf Extract of Fleurya aestuans in Diabetic Rats
Asian Research Journal of Gynaecology and Obstetrics,
Diabetes mellitus produces excessive reactive oxygen species (ROS) in the male gonads resulting to impairments of steroidogenesis and spermatogenesis. Fleurya aestuans is an extremely basic plant with therapeutic properties but it’s frequently viewed as a weed in Nigeria. The present study therefore attempts to examine the effects of the hydroethanolic leaf extract of Fleurya aestuans on reproductive dysfunctions of diabetic rats. The rats were randomly assigned into seven groups of five rats each. Diabetes was induced in all the test groups 2-7 by intra-peritoneal injection of 150mg/kg bwt of alloxan monohydrate. Rats of the first group served as control and were only allowed rat feed and tap water ad libitum. Rats of the second group served as alloxan only group (ALXOG) and received 150mg/kg bwt of alloxan monohydrate. The third, fourth and fifth groups served as low dose extract group (LDEG), medium dose extract group (MDEG) and high dose extract group (HDEG) and received 50, 75 and 200mg/kg bwt of the extract respectively. Rats of the sixth group served as positive control group 1 (PCG1) and received 600mg/kg bwt of glibenclamide while rats of the seventh group served as positive control group 2 (PCG2) and received 100mg/kg bwt of tetrahydroxyflavone. Both extract and drugs were administered orally using oral gavage. The results indicated that the extract significantly reduced (p<0.05) blood glucose levels (203.10±0.10, 136.06±0.06, 111.01±0.01, 106.01±0.08) in contrast to group 2 (399.02±0.02, 277.02±0.02, 291.02±0.02, 383.01±0.00). In addition, the extract significantly improved (p<0.05) conceptive hormones, sperm parameters, SOD, CAT and GSH parameters. Our study confirmed that the leaf extract of Fleurya aestuans can be utilized in the correction of male reproductive dysfunction mediated by diabetes.
- Fleurya aestuans
- conceptive hormones
- wistar rodents
How to Cite
Gyan Chand Jain and Ram Niwas Jangir. Modulation of diabetes-mellitus-induced male reproductive dysfunctions in experimental animal models with medicinal plants. Journal of Pharmacognosy Review. 2014;8(16):113–121.
Alves MG, Martins AD, Cavaco JE, Socorro S, Oliveira PF. Diabetes, insulin-mediated glucose metabolism and Sertoli/blood-testis barrier function. Tissue Barriers. 2013;1(e23992.8):42-46
La Vignera S, Condorelli R, Vicari E, D’Agata R, Calogero AE. Diabetes mellitus and sperm parameters. Journal of Andrology. 2012;33:145–53.
Schoeller EL, Schon S, Moley KH. The effects of type 1 diabetes on the hypothalamic, pituitary and testes axis. Cell Tissue Research. 2012;349:839–47.
Chien-Feng Mao, Sabri Sudirman, Chi-Chih Lee, David Tsou and Zwe-Ling Kong. Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats. Frontiers of Veterinary Science. 2021;651286.
Marín-Peñalver JJ, Martín-Timón I, Sevillano-Collantes C, Cañizo-Gómez FJD. Update on the treatment of type 2 diabetes mellitus. World Journal of Diabetes. 2016;7:354–95.
Ganiyat K, Oloyede, Olukoyej, E. Ayanbadejo.: Phytochemical, Toxicity, Antimicrobial and antioxidant Screening of Extracts Obtained from Fleurya aestuans (Gaud). Journal of Medical Sciences. 2014;14:51-59.
Tijani S Abiola, Opajobi Ifeoluwa C, Babalola OO. Protective effect of methanolic extract of laportea aestuans on indomethacin-induced kidney damage in male wistar rats. Journal of Environmental Research and Management. 2019;10(1): 005-011
Fagbohun AB, Fred Jaiyesimi AA, Adegboyega AA, Kasim LS, Kesi C3, Ndimele BE, Oluboba MA. Anti-diabetic activities of Fleurya aestuans (L.) Gaudich in alloxan induced rats. 2017; African Journal of Pharmacy and Pharmacology
Okereke SC, Elekwa I, Nmaju AU. Gas Chromatographic fid, hypoglycemic and hypolipidemic effects of leaves of laportea aestuans in alloxan induced diabetes in male albino rats. Journal of Environmental Science, Toxicology and Food Technology; 2014.
Charles CN, Amah-Tariah FS, Adienbo OM, Dapper DV. Ameliorative effects of fleurya aestuans leaves on mating behaviours and reproductive hormones of lead acetate induced ovarian toxicity. European Journal of Research in Medical Sciences. 2021;9(1). ISSN 2056-600X.
Sofowora A. Medicinal plants and traditional medicine in Africa. Ibadan, Nigeria. 2008; Spectrum Books Limited.
Kelly DA, Nelson R.characterization and quantification by gas chromatography of flavonoids. Journal of Braz Chemstry. 2014;25.
Mbaka GO, Adeyemi OO, Noronha CC, Anunobi CC, Okanlawon AO. Anti- hyperglycemic and hypoglycaemic effects of ethanol root extract of Sphenocentrumjollyanumin normal and alloxan induced diabetic rabbits. Brazilian Journal of Morphological Science. 2009; 26(2):123-127.
Ogbonnia SO, Mbaka GO, Adekunle A, Anyika EO, Gbolade OE, Nwakakwa N. Effect of poly-herbal formulation, Okudiabet on Alloxan – Induced diabetic rats. Agric and Biological Journal of North America. 2010;1(2):139–145.
Lorke D. A new approach to practical acute toxicity testing. Archieve Toxicology. 1983; 54(4):275-287.
Chan CH, Ngoh GC, Yusoff R. A brief review on anti-diabetic plants: Global distribution, active ingredients, extractiontechniques and acting mechanisms. Journal of Pharmacognosy Review. 2012;6:22–8.
Victor Udo Nna, Ainul Bahiyah Abu Bakar, Mahaneem Mohamed. Diabetes mellitus-induced male reproductive impairment: The role of natural products: Journal of Applied Pharmaceutical Science. 2017; (09):233-242.
Khaki A, Fathiazad F, Nouri M, Khaki A, Maleki NA, Khamnei HJ, Ahmadi P. Beneficial effects of quercetin on sperm parameters in streptozotocin‐induced diabetic male rats. Journal of Phytotherapy Research. 2010;24:1285-1291.
Kanter M, Aktas C, Erboga M: Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin-induced diabetic rat testis. Journal of Food Chemistry and Toxicology. 2012;50:719-725.
Roy S, Rahaman N, Ahmed F, Metya S, Sannigrahi S. Naringenin attenuates testicular damage, germ cell death and oxidative stress in streptozotocin induced diabetic rats: naringenin prevents diabetic rat testicular damage. Journal of Applied Biomedicine. 2013;11:195-208.
Giribabu N, Kumar KE, Rekha SS, Muniandy S, Salleh N. Chlorophytum borivilianum root extract maintains near normal blood glucose, insulin and lipid profile levels and prevents oxidative stress in the pancreas of streptozotocin-induced adult male diabetic rats. International Journal of Medical Science. 2014;11:1172-1184.
Ogunyinka BI, Oyinloye BE, Osunsanmi FO, Opoku AR, Kappo AP. Modulatory influence of Parkia biglobosa protein isolate on testosterone and biomarkers of oxidative stress in brain and testes of streptozotocin-induced diabetic male rats. International Journal of Physiology, Pathophysiology and Pharmacology. 2016;8:78-86.
National Institute of Health. Laboratory Animal Welfare. United State Department of Health and Human Services. 1985;14:8.
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