Abortifacient Properties of Ethanolic Leaf Extract of Jatropha curcas Linn. in Female Wistar Rats

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Augustine Ikhueoya Airaodion
Emmanuel Onyebuchi Ogbuagu


Aim: This study sought to investigate the abortifacient properties of Jatropha curcas leaf extract in female Wistar rats.

Methods: Healthy leaves of J. curcas were harvested, dried and extracted using soxhlet apparatus and ethanol as the solvent. Thirty fertile male and thirty female Wistar rats were acclimatized for seven days. The females were separated into individual cages and had estrus synchronization using Diethylstilbestrol dissolved in paraffin oil and administered at the dose of 1 mg/kg body weight. A male rat was then introduced into each cage for mating. After confirmation of pregnancy, the pregnant rats were grouped into four. Group A was treated with normal saline, groups B, C and D were treated with 1 g/kg body weight of J. curcas leaf extract for 24, 48 and 72 hours respectively. The animals were then observed daily if they littered. In vitro effect of the fruit on isolated rats’ uteri was determined using standard method.

Results: J. curcas leaf extract was safe in rats at the tested oral doses (500–2000 mg/kg). There was no mortality within the study period. In the in vitro experiment, J. curcas leaf extract elicited a dose dependent multiple contractions on the pregnant rats’ uteri. 60% of animals treated with J. curcas leaf extract for 24 hours were observed to heave littered which is suggestive that miscarriage had occurred in 40%. In the animals treated for 48 hours, only 20% littered which is also suggestive that miscarriage had occurred in the remaining 80%. In the group treated with J. curcas leaf extract for 72 hours, no animal littered.

Conclusion: The results from this study revealed that J. curcas leaf extract induced multiple contractions on pregnant rats’ uteri following in vitro and in vivo administrations. This is an indication that it contains active agents which could be isolated and processed into pure utero-tonic agents. Therefore, care should be taken in the use of J. curcas leaves during pregnancy and its use in folklore medicine during pregnancy should be discouraged. However, it might serve as an effective abortifacient.

Abortifaceous properties, Jatropha curcas, miscarriage, oxytocin, pregnancy

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How to Cite
Airaodion, A. I., & Ogbuagu, E. O. (2020). Abortifacient Properties of Ethanolic Leaf Extract of Jatropha curcas Linn. in Female Wistar Rats. Asian Research Journal of Gynaecology and Obstetrics, 4(1), 1-8. Retrieved from https://journalarjgo.com/index.php/ARJGO/article/view/30128
Original Research Article


1. Robinson GE. Pregnancy loss: Best Practice & Research. Clinical Obstetrics & Gynaecology. 2014;28(1):169–178.
2. Airaodion AI, Ekenjoku JA, Ogbuagu EO, Okoroukwu VN, Ogbuagu U. Carica papaya leaves might cause miscarriage. Asian Research Journal of Gynaecology and Obstetrics. 2019;2(2):1-9.
3. Paul A, Fitzgerald MD, Klonoff DC. Endocrine drugs in basic and clinical pharmacology Ed. Bertram G. Katzung. 1998;603-616.
ISBN: 0-8385-0592-9
4. Gimpl G, Fahrenholz F. Oxytocin receptor system: Structure, function, and regulation Physiol Rev. 2001;81:629–83.
5. Ganong WF. Central regulation of visceral functions in Review of Medical Physiology 16th Edition 1993;217-222 & 411-412.
ISBN: 0-8385-8234-6.
6. Huffmeijer R, Alink LR. Tops M. Salivary levels of oxytocin remain elevated for more than two hours after intranasal oxytocin administration. Neuro Endocrinol. Lett. 2012;33(1):21–25.
7. Granner DK. Pituitary and Hypothalamic Hormones in Harper‟s Biochemistry Ed. 2000;550-558.
ISBN: 0-8385-3684-0
8. Marieb EN. Pregnancy and Human Development in Human Anatomy and Physiology 1992;974-994.
ISBN: 0-8053-4120-X
9. Edeoga HO, Okwu DE, Mbaebre BO. Phytochemical constituents of some Nigerian plants. African Journal of Biotechnology. 2005;447:685-688.
10. Duke JA, Edward SA. Medicinal plants of China. Michigan Reference Publications; 1985.
11. Agbogidi OM, Mariere AE, Ohwo A. Metal concentration in plant tissues of Jatropha curcas L grown in crude oil contaminated soil. Journal of Bioinnovation. 2013;2(3): 137-145.
12. Kumar A, Ashwani CA, Satyawati S. 2008. An evaluation of multipurpose oil seed crop for industrial uses Jatropha curcas L.: A review. Industrial Crops and Products. 2008;1-8.
13. Agbogidi OM, Akparobi SO, Eruotor PG. Health and environmental benefits of jatropha curcas Linn. Applied Science Reports. 2013;1(2):36-39.
14. Chehregani A, Malauer BE. Removal of heavy metals by native accumulates plants. World Journal of Agricultural Sciences. 2007;8:7-10.
15. Aliyu BS. Some ethnomdicinal plants of the Savannah regions of West Africa: description and phytochemicals. Triumph Publishing Company Ltd, Kano; 2006.
16. Okoli RI, Aigbe O, Ohafu-Obode JO, Mensah JK. Medicinal herbs used for managing some common ailments among Esan people Edo State, Nigeria. Pakistan Journal of Nutrition. 2007;6(5):470-490.
17. Prminik K. Properties and use of Jatropha curcas oil and diesel fuel blends compression ignition engines. Renewable Energy. 2002;28:239-248.
18. Nayak BS, Patel KN. Pharmacognosis studies of Jatropha curcas leaves. International Journal of Pharmtech Research. 2010;2(1):140-143.
19. Airaodion AI, Ogbuagu EO, Airaodion EO, Ekenjoku JA, Ogbuagu U. Pharma-cotherapeutic effect of methanolic extract of Telfairia occidentalis leaves on glycemic and lipidemic indexes of alloxan-induced diabetic rats. International Journal of Bio-Science and Bio-Technology. 2019;11(8): 1-17.
20. Airaodion AI, Ogbuagu EO, Ekenjoku JA, Ogbuagu U, Airaodion EO. Therapeutic effect of methanolic extract of Telfairia occidentalis leaves against acute ethanol-induced oxidative stress in Wistar rats. International Journal of Bio-Science and Bio-Technology. 2019;11(7):179-189.
21. Airaodion AI, Okoroukwu VN, Ogbuagu EO, Ogbuagu U. In vitro and in vivo evaluation of Ananas comosus fruit (pineapple) on abortion/miscarriage in wistar rats. International Journal of Bio-Science and Bio-Technology. 2019;11(9): 69-75.
22. Airaodion AI, Ogbuagu EO, Okoroukwu VN, Ekenjoku JA, Ogbuagu U, Airaodion EO. Does Chrysophyllum albidum fruit (cherry) induce abortion/miscarriage or not. International Journal of Research and Reports in Gynaecology. 2019;2(1):1-7.
23. Alwi NA, Sukardi S. Anti-implantation effects of Jatropha curcas crude oil when fed to pregnant sprague dawley rats during the early gestation period. Advances in Life Science and Technology. 2013;14:1-7.
24. Farnsworth MR, Bingel AS, Cordell GA, Crane FA, Fong HS. Potential value of plants as sources of new anti-fertility agent. J. Pharm. 1 Sci. 1975;64(4):535-598.
25. Falodun A, Nworgu ZAM, Osayemwenre E. Smooth muscle relaxant evaluation of Jatropha Curcas Linn (euphorbiaceae) and isolation of triterpenes. Nigerian Journal of Physiological Science. 2011;133–137.
26. Xiao CW, Murphy BD, Sirois J. Goff AK. Downregulation of oxytocin-induced cyclooxygenase-2 and prostaglandin F2∞ Synthase expression by interferon-T in Endometrial cells. Boil. Reprod. 1999;60: 656-663.
27. Alexandria AB, Soloff HE. The effect of calcium deposit on smooth Muscles Reprod. Genet. 1979;14(9):495-496.