Effect of Clomiphene Citrate on the Hormonal Profile of Adult Female Wistar Rats with Regular Estrous Cycle

M. A. Olugbenga *

Department of Anatomy, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Nigeria.

O. O. Oseni

Department of Anatomy, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Nigeria.

W. O. Ohiwerei

Department of Research and Training, Ohilux Research and Training Institute, Ekpoma, Edo State, Nigeria.

Christian Ugege

Edo Specialist Teaching Hospital, Benin City, Edo State, Nigeria.

A. O. Lauretta

Paxxmed Diagnostic Service, Abuja, Nigeria.

A. P. Eidangbe

Department of Chemical Pathology, Faculty of Medical Laboratory Science, Ambrose Alli University Ekpoma, Edo State, Nigeria.

S. Ehimare

Department of Chemical Pathology, Irrua Specialist Teaching Hospital, Irrua, Edo State, Nigeria.

J. P. Akhaine

Central Hospital, Benin City, Edo State, Nigeria.

O. F. Ohiwerei

Department of Research and Training, Ohilux Research and Training Institute, Ekpoma, Edo State, Nigeria.

*Author to whom correspondence should be addressed.


This study investigates the effect of Clomiphene citrate (CC) on the Hormonal profile of female Adult Wistar rats with regular estrous cycle. A total of thirty six (36) healthy female Wistar rats were reared and screened for the purpose of this study. The animals were divided into 4 groups consisting of 4 rats each with regular estrous cycle and were treated as follows; Group 1 served as control while Groups 2, 3 and 4 were treated with 1 mg/kg, 2mg/kg and 5 mg/kg body weight of CC respectively, for 11 consecutive days. Body weight was obtained at commencement and every other day of the experiment using digital weighing scale. There was no statistically significant (p>0.05) difference in body weight of rats with regular estrous cycle at day 1, 3, 5 and 7 compared to control. However, there was a statistically significant (p<0.05) difference in body weight across the group on day 9 and 11. Progesterone was significantly (p<0.05) higher in the 1mg as well as 5mg CC treated groups with a significant decrease in follicle stimulating hormone (FSH) value across treatments. CC could improve the sex hormone changes in women due to its anti-estrogenic properties, which is consistent with our results hence, the significant changes observed in the reproductive hormone levels. 

Keywords: Clomiphene citrate (CC), progesterone, follicle stimulating hormone (FSH)

How to Cite

Olugbenga , M. A., Oseni , O. O., Ohiwerei , W. O., Ugege , C., Lauretta , A. O., Eidangbe , A. P., Ehimare , S., Akhaine , J. P., & Ohiwerei , O. F. (2023). Effect of Clomiphene Citrate on the Hormonal Profile of Adult Female Wistar Rats with Regular Estrous Cycle. Asian Research Journal of Gynaecology and Obstetrics, 6(1), 100–107. Retrieved from https://journalarjgo.com/index.php/ARJGO/article/view/175


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